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[6-12]Five-Dimensional Single Particle Tracking Reveals Rotational Motions in Live Cells

发布者:金雪明发布时间:2019-06-10浏览次数:10

报告题目:Five-Dimensional Single Particle Tracking Reveals Rotational Motions in Live Cells

报 告 人:方宁 副教授(美国佐治亚州立大学)

主 持 人:张秀莉 教授

报告地点:苏州大学独墅湖二期云轩楼1323

报告时间:2019612日(周三)上午1000

    

  

报告人简介

1998年毕业于厦门大学,2006年在加拿大不列颠哥伦比亚大学获得博士学位,师从David D.Y Chen教授。2006-2008年在爱荷华州立大学和美国能源部Ames实验室的Edward S. Yeung教授课题组进行博士后研究。从2008年至2015年,他是爱荷华州立大学化学助理教授,美国能源部Ames实验室的科学家。20157月,他被佐治亚州立大学化学系聘为副教授。其研究方向为基于新型光学成像平台的化学、生物和药学研究。其发展的光学成像平台能提供超衍射极限的空间分辨率和超高的角度分辨率,可以实现单分子和纳米颗粒的纳米级精度定位。已在Nano Today, Nat. Catal., Nat. Commun., PNAS, Chem. Rev. Angew. Chem. Int. Ed., J. Am. Chem. Soc., Nano Lett.等杂志发表近百篇学术论文。

  

报告摘要

The knowledge of rotational dynamics in and on live cells remains highly limited due to technical limitations. The Single Particle Orientation and Rotational Tracking (SPORT) techniques have been developed in the Fang Laboratory to acquire accurate measurements of anisotropic plasmonic gold nanorods in complex cellular environments. Rich information in five dimensions, including the x, y, z coordinates and the two orientation angles (azimuthal angle j and polar angle q , as defined in the figure) of the probe’s transition dipole, can be obtained from SPORT experiments. The SPORT technique is capable of extracting important information (including rotational rates, modes, and directions) on the characteristic rotational dynamics involved in cellular processes, such as adhesion, endocytosis, and transport of functionalized nanoparticles, as may be relevant to drug delivery and viral entry.